Journal: PLoS ONE

Article Title: Metabolism of AGEs – Bacterial AGEs Are Degraded by Metallo-Proteases

doi: 10.1371/journal.pone.0074970

Figure Lengend Snippet: Lysate was extracted from exponentially growing cultures and further separated into high and low-molecular-mass fractions, as described in Materials and Methods. The high molecular weight fraction was treated and incubated at 37°C for 3 hours and then separated by size using HiLoad 16/600 Superdex 75 pg 120 ml filtration column. After 30 ml of elution, 4 ml fractions were collected and AGEs specific fluorescence was determined. The graph represents the size distribution of AGEs in an untreated sample, lystae treated with 3M urea and lystae treated with protease inhibitor cocktail (Sigma).

Article Snippet: For AGEs size exclusion filtration measurements ( ) samples were separated using GE HiLoad 16/600 Superdex 75 pg 120ml filtration column, equilibrated with PBS.

Techniques: Molecular Weight, Incubation, Filtration, Fluorescence, Protease Inhibitor

Journal: PLoS ONE

Article Title: Metabolism of AGEs – Bacterial AGEs Are Degraded by Metallo-Proteases

doi: 10.1371/journal.pone.0074970

Figure Lengend Snippet: Effect of 100 µM 1,10 phenantholine (Sigma) on AGEs degradation. Bacteria were grown as described in materials and methods. Lysate was extracted from treated and untreated cells and further separated by size using HiLoad 16/600 Superdex 75 pg 120 ml filtration column. After 30 ml of elution, 4 ml fractions were collected and AGEs specific fluorescence was determined.

Article Snippet: For AGEs size exclusion filtration measurements ( ) samples were separated using GE HiLoad 16/600 Superdex 75 pg 120ml filtration column, equilibrated with PBS.

Techniques: Filtration, Fluorescence

Journal: bioRxiv

Article Title: Tuning of granulopoietic signaling by de novo designed agonists

doi: 10.1101/2023.11.25.568662

Figure Lengend Snippet: (A) Preparative size exclusion chromatography of Boskar4 short tandem fusion (st2) variants after Nickel IMAC on two separate SEC-columns of the same type (HiLoad 16/600, Superdex 200 pg). Dotted lines mark the molecular weight standard for the corresponding column. All fractions of the main peak (black box) were collected and concentrated by ultrafiltration (10 kDa cut-off). (B) A representative sample of each protein and a protein molecular weight marker (M) (11852124, Thermo Fisher Scientific) was loaded on a SDS-PAGE (18% PA, 150 V, 1 h) which was subsequently stained with Coomassie. The expected molecular weight of Boskar4_st2 and variants (with not cleaved TEV-cleavage site and His-tag) is ∼29 kDa.

Article Snippet: Finally, size exclusion chromatography (SEC) was performed with PBS on the concentrated sample with a HiLoad 16/600, Superdex 200 pg (GE28-9893-35, Merck) column or other if mentioned in the corresponding legend description.

Techniques: Size-exclusion Chromatography, Molecular Weight, Marker, SDS Page, Staining

Journal:

Article Title: Crystallization and preliminary crystallographic studies of putative threonyl-tRNA synthetases from Aeropyrum pernix and Sulfolobus tokodaii

doi: 10.1107/S1744309108026924

Figure Lengend Snippet: Protein purification and concentration procedures The columns used were from the following makers: Q-Sepharose Fast Flow, Superdex200, Resource ISO, Resource Q, HiLoad 16/60 Superdex75, HiLoad 16/60 Superdex200 and HiTrap Heparin HP were from Amersham Biosciences, Hydroxyapatite Type 1, BioScale CHT5I and Bioscale CHT10I were from Bio-Rad, Phenyl-Toyopearl Pak650S was from Tosoh and DEAE-celluose A500-sf from Seikagaku Biobusiness Corp. The concentrations, filter devices and buffers for the native and selenomethione-labelled protein pairs were identical. β-ME, β-­mercaptoethanol.

Article Snippet: Third column , HiLoad 16/60 Superdex75 , , BioScale CHT5I , HiTrap Heparin HP.

Techniques: Protein Purification, Concentration Assay